Appearance of iron-labeled blood mononuclear cells in electron microscopy

To chci

Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM (transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes. … celý popis

Podrobná bibliografie

Další autoři:

Drahomír Horký, 1939- , Irena Lauschová, 1956- , M. Klabusay, M. Doubek, Peter Scheer, 1973- , S. Palsa, Jaroslav Doubek, 1947-

Typ dokumentu:

Články

Publikováno v:

Veterinární medicína. -- ISSN 0375-8427. -- Roč. 51, č. 3 (2006), s. 89-92

Témata:

laboratorní zvířata

králíci

kostní dřeň

krvinky

leukocyty

monocyty

lymfocyty

buněčná kultura

železo

ultrastruktura

mikroskopie

LABORATORY ANIMALS

RABBITS

BONE MARROW

BLOOD CELLS

LEUKOCYTES

MONOCYTES

LYMPHOCYTES

CELL CULTURE

IRON

ULTRASTRUCTURE

MICROSCOPY

mononukleární buňky

označování

nanočástice

mikroskopie elektronová

animal model

Popis jednotky:

fot.

Fyzický popis:

6 il.

Bibliografie:

Bibliografie na s. 92 (5 zázn.)