Problems and possibilities of monocot transformation

To chci

Biotechnological improvement of monocots is often hampered by the lack of efficient regeneration systems, requisite wound responses and low cell competence. Despite these limitatations, the biolistic and Agrobacterium methods have been successfully used to produce everal transgenic monocots by adjusting the parameters that govern efficient delivery and integration of transgene(s) into plant genome. It is now possible to transform even difficult monocots using tailor-made gene constructs and promoters, suitable A. tumefaciens strains and a proper understanding of the entire process. This success has been reviewed in the present article and a special emphasis was laid on the measures that were taken in overcoming the difficulties that arise due to the differential responses of monocots and dicots. This information is necessary for biotechnological improvement of still newer monocotyledonous plants that have been hitherto difficult to transform. … celý popis

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Hlavní autor:

P. Sood

Další autoři:

Anirban Bhattacharya
A. Sood

Typ dokumentu:

Články

Publikováno v:

Biologia plantarum. -- ISSN 0006-3134. -- Vol. 55, no. 1 (2011), s. 1-15

Témata:

jednoděložné rostliny

transgenoze

genový přenos

Agrobacterium

microprojectile bombardment

tailor-made gene constructs

mikroprojektilový přenos DNA

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